synoviocytes hflss  (Cell Applications Inc)

Journal: Communications Biology

Article Title: METTL3-mediated fibroblast-like synoviocytes senescence promotes temporomandibular joint osteoarthritis progression

doi: 10.1038/s42003-026-09773-x

Figure Lengend Snippet: BLM-treated hFLSs were transfected with shMETTL3-1 (IME4-1) or shMETTL3-2 (IME4-2). A , B The transfection efficiency of shMETTL3-1 and shMETTL3-2 was verified by Western blotting. C , D Verification of cellular senescence with a β-GAL kit. E TEM for observing mitochondrial autophagosomes. F , G TMRM staining. H , I Western blotting data of P62 expression in the extracted mitochondria. J – P Western blotting analysis of p16 INK4a , p21, DcR2, PINK1, and Parkin. Q Double IF staining results for LC3B and TOMM20. *** P < 0.001 vs. the CTRL group; ## P < 0.01, ### P < 0.001 vs. the BLM+shCTRL group.

Article Snippet: Human fibroblast-like synoviocytes (hFLSs) were obtained from Cell Applications, Inc. (No. 408-05a, San Diego, CA, USA) and maintained in HFLS medium (No. 415–500, Cell Applications) at 37 °C with 5% CO2, as previously described .

Techniques: Transfection, Western Blot, Staining, Expressing

Journal: Communications Biology

Article Title: METTL3-mediated fibroblast-like synoviocytes senescence promotes temporomandibular joint osteoarthritis progression

doi: 10.1038/s42003-026-09773-x

Figure Lengend Snippet: The m 6 A levels of PINK1 mRNA were confirmed by m6A RIP-qPCR in the synovial tissues of sham and MIA-induced TMJOA rats ( A ), primary FLSs from sham and MIA-induced TMJOA rats ( B ), BLM-treated hFLSs ( C ), and METTL3-silenced hFLSs after treatment with BLM ( D ), ** P < 0.01; *** P < 0.001. E Potential m6A sites for PINK1 mRNA. MeRIP-qPCR analysis of PINK1 m 6 A mRNA levels at the 2028 ( F ), 2074 ( G ), 2174 ( H ), and 2483 ( I ) sites in hFLSs after transfection with shMETTL3 (IME4), ** P < 0.01; *** P < 0.001. J After IME4 silencing, the level of PINK1 was monitored via RT-qPCR in BLM-induced hFLSs treated with 2.5 μM actinomycin D for 0, 4, 8, and 12 h. The estimated half-life of PINK1 mRNA was 7.83 h in the BLM group and 11.33 h after IME4 knockdown. Data are mean ± SD from three independent experiments. *** P < 0.001 vs. the CTRL group; ### P < 0.001 vs. the BLM group.

Article Snippet: Human fibroblast-like synoviocytes (hFLSs) were obtained from Cell Applications, Inc. (No. 408-05a, San Diego, CA, USA) and maintained in HFLS medium (No. 415–500, Cell Applications) at 37 °C with 5% CO2, as previously described .

Techniques: Transfection, Quantitative RT-PCR, Knockdown

Journal: Communications Biology

Article Title: METTL3-mediated fibroblast-like synoviocytes senescence promotes temporomandibular joint osteoarthritis progression

doi: 10.1038/s42003-026-09773-x

Figure Lengend Snippet: BLM-induced hFLSs were transfected with shMETTL3 or shPINK1. A , B Validation of PINK1 protein expression. C , D Assessment of cellular senescence with a β-GAL kit. E TEM images. F , G TMRM staining. H , I P62 expression in mitochondria. J – M Western blotting analysis of Drp1, Mfn2, p16 INK4a , p21, and DcR2 expression. N Double IF staining of LC3B and TOMM20 expression. *** P < 0.001.

Article Snippet: Human fibroblast-like synoviocytes (hFLSs) were obtained from Cell Applications, Inc. (No. 408-05a, San Diego, CA, USA) and maintained in HFLS medium (No. 415–500, Cell Applications) at 37 °C with 5% CO2, as previously described .

Techniques: Transfection, Biomarker Discovery, Expressing, Staining, Western Blot